Prevalence and Genetic Diversity of Escherichia coli Isolated from Sewage Samples of Hospitals in Different Regions of Bangladesh

Escherichia coli are a large and diverse group of bacteria found in the environment, foods intestines humans animals. This study was carried out to observe genotypic variations E. at different regions Bangladesh using Amplified Ribosomal DNA Restriction Analysis (ARDRA) 16S rDNA RAPD (Random Polymorphic DNA). Sewage samples were collected from medical college hospitals total 118 observed this which 26 identified as based on their cultural, biochemical molecular characteristics. Antibiotic susceptibility test where most isolates resistant Amoxicillin, Rifampicin Erythromycin; some kanamycin, ceftriaxone, Chloramphenicol Azithromycin; besides 73% (N=19 26) multi-drug resistant. Results Polymerase chain reaction (PCR) showed that none Stx1, Stx2 LT gene. Two groups by Alu1 restriction enzyme seven Random Amplification (RAPD) patterns found. After analyzing, it 24 similarity with strain NBRC 102203 remaining two Mui T5 ARDRA sequences rDNA. From study, concluded more prevalent than isolated sewage sample Bangladesh.
 J Microbiol, Volume 38, Number 2, December 2021, pp 39-44